2H4V
Crystal Structure of the Human Tyrosine Receptor Phosphatase Gamma
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-05-06 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97925 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 74.849, 78.859, 121.758 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.230 - 1.550 |
R-factor | 0.16233 |
Rwork | 0.161 |
R-free | 0.18130 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2fh7 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.411 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.230 | 1.630 |
High resolution limit [Å] | 1.550 | 1.550 |
Number of reflections | 105096 | |
Completeness [%] | 100.0 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | Ammonium acetate, citrate, PEG4K, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |