2H4Q
Crystal structure of a M-loop deletion variant of MENT in the cleaved conformation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ELLIOTT GX-13 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-04-07 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5148 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 150.194, 150.194, 175.485 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.350 - 2.100 |
| R-factor | 0.189 |
| Rwork | 0.188 |
| R-free | 0.21300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qlp |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.874 |
| Data reduction software | CrystalClear ((MSC/RIGAKU)) |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 105.400 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.062 | 0.587 |
| Number of reflections | 44451 | |
| <I/σ(I)> | 20.7 | 2.4 |
| Completeness [%] | 99.3 | 94.6 |
| Redundancy | 3.4 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 290 | 0.1M sodium acetate, 12-15% PEG 8000, pH 5.5-6.5, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
