2H1L
The Structure of the Oncoprotein SV40 Large T Antigen and p53 Tumor Suppressor Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2005-07-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | .980 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 127.019, 182.718, 262.061 |
| Unit cell angles | 90.00, 92.08, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.160 |
| R-factor | 0.069 |
| Rwork | 0.261 |
| R-free | 0.30810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1svm |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.213 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.270 |
| High resolution limit [Å] | 3.160 | 3.160 |
| Number of reflections | 204669 | |
| <I/σ(I)> | 30.3 | 4.5 |
| Completeness [%] | 97.5 | 94.3 |
| Redundancy | 3.7 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 298 | 5.4% PEG 4000, 73mM Mops Buffer, 22mM Lithium Sulfate, pH 6.5, VAPOR DIFFUSION, temperature 298K |
