2H1K
Crystal structure of the Pdx1 homeodomain in complex with DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-06-22 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 58.282, 61.928, 96.445 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 53.000 - 2.420 |
| Rwork | 0.227 |
| R-free | 0.27700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 9ant |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.570 |
| Data reduction software | MOSFLM |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.000 | 2.570 |
| High resolution limit [Å] | 2.420 | 2.420 |
| Rmerge | 0.079 | 0.351 |
| Number of reflections | 14198 | |
| <I/σ(I)> | 2.1 | |
| Completeness [%] | 99.6 | 97.6 |
| Redundancy | 5.3 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | microbatch-under-oil | 7 | 281.15 | 0.1 mM Imidazole pH 7.0, 280 mM MgCl2, and 21.4 % PEG 4000, microbatch-under-oil, temperature 281.15K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Imidazole | ||
| 2 | 1 | 1 | MgCl2 | ||
| 3 | 1 | 1 | PEG 4000 | ||
| 4 | 1 | 2 | Imidazole | ||
| 5 | 1 | 2 | MgCl2 | ||
| 6 | 1 | 2 | PEG 4000 |
