2GZH
Crystal Structure of Rab11 in Complex with Rab11-Family Interacting Protein 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Detector technology | CCD |
| Collection date | 2005-12-06 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.98175 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 64.740, 64.740, 112.400 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.470 |
| R-factor | 0.19914 |
| Rwork | 0.197 |
| R-free | 0.25353 |
| Structure solution method | SAD |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.977 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.560 |
| High resolution limit [Å] | 2.470 | 2.470 |
| Rmerge | 0.067 | 0.297 |
| Number of reflections | 10234 | |
| <I/σ(I)> | 16.3 | 15.9 |
| Completeness [%] | 99.8 | 98.6 |
| Redundancy | 16 | 10 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5 | 298 | Ammonium phosphate buffer, approximately 0.3M concentration, pH 5, VAPOR DIFFUSION, temperature 298K |
