2GYY
Structure of aspartate semialdehyde dehydrogenase (ASADH) from Streptococcus pneumoniae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2005-10-01 |
Detector | RIGAKU RAXIS IV |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 72.479, 78.686, 243.410 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.110 - 2.100 |
R-factor | 0.18 |
Rwork | 0.178 |
R-free | 0.22100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | : 1YS4 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.104 |
Data reduction software | CrystalClear ((MSC/RIGAKU)) |
Data scaling software | d*TREK (9.2SSI) |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.120 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.085 | 0.254 |
Total number of observations | 28306 | |
Number of reflections | 77734 | |
<I/σ(I)> | 8 | 3.9 |
Completeness [%] | 94.6 | 99.9 |
Redundancy | 3.27 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 100 mM MES pH 6.5 50 mM calcium acetate 20% PEG 3350 10 mM DTT, VAPOR DIFFUSION, HANGING DROP, temperature 293K |