2GY5

Tie2 Ligand-Binding Domain Crystal Structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2005-07-01
Detector	ADSC QUANTUM 315
Wavelength(s)	1.215
Spacegroup name	P 41 21 2
Unit cell lengths	114.653, 114.653, 113.774
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.900
R-factor	0.2599
Rwork	0.240
R-free	0.29400
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.632
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CNS (1.0)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.800
Number of reflections	96910
Completeness [%]	93.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	2.2M Ammonium Sulfate, 0.1M MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K