2GX5
N-terminal GAF domain of transcriptional pleiotropic repressor CodY
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-12-02 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97570 |
| Spacegroup name | P 43 2 2 |
| Unit cell lengths | 90.207, 90.207, 205.634 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.890 - 1.740 |
| R-factor | 0.18872 |
| Rwork | 0.187 |
| R-free | 0.21595 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2b18 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.076 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.800 |
| High resolution limit [Å] | 1.740 | 1.740 |
| Rmerge | 0.083 | 0.612 |
| Number of reflections | 85808 | |
| <I/σ(I)> | 22.11 | 1.67 |
| Completeness [%] | 98.0 | 95.2 |
| Redundancy | 7.2 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 291 | ammonium sulphate, sodium citrate, PEG 400, 20mM cGMP, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
