2GWR
Crystal structure of the response regulator protein mtrA from Mycobacterium Tuberculosis
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Wavelength(s) | 0.97972, 0.97952, 0.91840 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 38.916, 56.598, 135.069 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.309 - 2.100 |
R-factor | 0.204 |
Rwork | 0.204 |
R-free | 0.24400 |
Structure solution method | MAD |
RMSD bond length | 0.014 |
RMSD bond angle | 1.229 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.107 | 0.395 |
Number of reflections | 24268 | |
<I/σ(I)> | 11.4 | 3.4 |
Completeness [%] | 99.6 | 98.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8 | 277 | 10 mM Tris, 100 mM NaCl, 1mM beta-mercaptoethanol, pH 8.0, temperature 277K | |
1 | 8 | 277 | 10 mM Tris, 100 mM NaCl, 1mM beta-mercaptoethanol, pH 8.0, temperature 277K | |
1 | 8 | 277 | 10 mM Tris, 100 mM NaCl, 1mM beta-mercaptoethanol, pH 8.0, temperature 277K |