2GWF

Structure of a USP8-NRDP1 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2006-01-21
Detector	ADSC QUANTUM 210
Wavelength(s)	0.9800
Spacegroup name	P 1 21 1
Unit cell lengths	76.111, 67.094, 99.275
Unit cell angles	90.00, 91.12, 90.00

Refinement procedure

Resolution	30.000 - 2.300
R-factor	0.20767
Rwork	0.206
R-free	0.23704
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	1.191
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.380
High resolution limit [Å]	2.300	2.300
Number of reflections	41906
<I/σ(I)>	21.98	3.15
Completeness [%]	93.4	97.8
Redundancy	4	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	12% PEG5000 MME, 0.1 M bis-Tris, 1 mM DTT, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K