2GWD
Crystal structure of plant glutamate cysteine ligase in complex with Mg2+ and L-glutamate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-12-13 |
Detector | Large Area Detector |
Wavelength(s) | 0.9782 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 54.810, 54.810, 518.040 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.460 - 2.090 |
R-factor | 0.178 |
Rwork | 0.175 |
R-free | 0.22670 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2GWC monomer |
RMSD bond length | 0.012 |
RMSD bond angle | 1.314 |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.460 | 2.220 |
High resolution limit [Å] | 2.090 | 2.090 |
Number of reflections | 27931 | |
<I/σ(I)> | 27.2 | 5.7 |
Completeness [%] | 94.8 | 70.5 |
Redundancy | 11 | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 18% PEG 3350, 0.2 M magnesium acetate tetrahydrate, 0.1 M tricine, 0.05 M L-glutamate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |