2GU5
E. coli methionine aminopeptidase in complex with NleP, 1: 1, di-metalated
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2005-08-21 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 49.790, 64.606, 76.354 |
Unit cell angles | 90.00, 107.77, 90.00 |
Refinement procedure
Resolution | 14.194 - 1.600 |
R-factor | 0.219 |
Rwork | 0.211 |
R-free | 0.23300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1xnz |
RMSD bond length | 0.004 |
RMSD bond angle | 1.295 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | CNS |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 20.000 | 14.200 | 1.690 |
High resolution limit [Å] | 1.600 | 5.060 | 1.600 |
Rmerge | 0.044 | 0.025 | 0.240 |
Total number of observations | 6966 | 29200 | |
Number of reflections | 60478 | ||
<I/σ(I)> | 14.1 | 18.8 | 3.1 |
Completeness [%] | 99.3 | 95.3 | 97.9 |
Redundancy | 3.6 | 3.7 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 15% PEG 20000, 0.1 M MES (pH 6.5) , VAPOR DIFFUSION, HANGING DROP, temperature 291K |