2GU5
E. coli methionine aminopeptidase in complex with NleP, 1: 1, di-metalated
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-08-21 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.790, 64.606, 76.354 |
| Unit cell angles | 90.00, 107.77, 90.00 |
Refinement procedure
| Resolution | 14.194 - 1.600 |
| R-factor | 0.219 |
| Rwork | 0.211 |
| R-free | 0.23300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xnz |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.295 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 20.000 | 14.200 | 1.690 |
| High resolution limit [Å] | 1.600 | 5.060 | 1.600 |
| Rmerge | 0.044 | 0.025 | 0.240 |
| Total number of observations | 6966 | 29200 | |
| Number of reflections | 60478 | ||
| <I/σ(I)> | 14.1 | 18.8 | 3.1 |
| Completeness [%] | 99.3 | 95.3 | 97.9 |
| Redundancy | 3.6 | 3.7 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 15% PEG 20000, 0.1 M MES (pH 6.5) , VAPOR DIFFUSION, HANGING DROP, temperature 291K |
