2GTF
Crystal structure of nitrophorin 2 complex with pyrimidine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-11 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 34.374, 34.374, 255.820 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.400 - 1.400 |
| R-factor | 0.1714 |
| Rwork | 0.169 |
| R-free | 0.21058 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1pee |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.895 |
| Data reduction software | XDISPLAYF |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.400 | 1.450 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Number of reflections | 29002 | |
| <I/σ(I)> | 32 | 2 |
| Completeness [%] | 91.0 | 73 |
| Redundancy | 5 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | sodium citrate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
