2GT2
Structure of the E. coli GDP-mannose mannosyl hydrolase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 200 |
Detector technology | CCD |
Collection date | 2004-10-10 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.0 |
Spacegroup name | P 32 |
Unit cell lengths | 48.767, 48.767, 210.230 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 223.610 - 2.000 |
R-factor | 0.204 |
Rwork | 0.201 |
R-free | 0.25700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1rya |
RMSD bond length | 0.007 |
RMSD bond angle | 1.058 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 223.610 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.070 | 0.206 |
Number of reflections | 34871 | |
<I/σ(I)> | 19.4 | 4.03 |
Completeness [%] | 92.3 | 63.8 |
Redundancy | 4.1 | 1.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.4M Potassium Sodium Tartrate tetra-hydrate, pH 0.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |