2GSM
Catalytic Core (Subunits I and II) of Cytochrome c oxidase from Rhodobacter sphaeroides
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 5ID-B |
| Synchrotron site | APS |
| Beamline | 5ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-30 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 125.020, 131.639, 176.802 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.21393 |
| Rwork | 0.214 |
| R-free | 0.23242 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.253 |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.051 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.060 | 0.399 |
| Number of reflections | 184839 | |
| <I/σ(I)> | 14.8 | 3.8 |
| Completeness [%] | 96.1 | 97.4 |
| Redundancy | 4.5 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.3 | 277 | PEG-400, pH 6.3, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
