2GSG
Crystal structure of the Fv fragment of a monoclonal antibody specific for poly-glutamine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-11-05 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9537 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 162.876, 78.096, 50.697 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.100 |
R-factor | 0.229 |
Rwork | 0.229 |
R-free | 0.25300 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.444 | |
Number of reflections | 37635 | |
<I/σ(I)> | 31.5 | 3.2 |
Completeness [%] | 96.6 | 86.4 |
Redundancy | 5.5 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 6.5 | 290 | 0.1M sodium cacodylate, pH 6.5, 20% PEG 8000, 0.2M (NH4)2SO4, EVAPORATION, temperature 290K |