2GSE
Crystal Structure of Human Dihydropyrimidinease-like 2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 173 |
Detector technology | CCD |
Collection date | 2006-02-12 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.933 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 86.400, 126.100, 102.900 |
Unit cell angles | 90.00, 113.00, 90.00 |
Refinement procedure
Resolution | 29.310 - 2.400 |
R-factor | 0.173 |
Rwork | 0.169 |
R-free | 0.24500 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.015 |
RMSD bond angle | 1.567 |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.600 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.143 | 0.430 |
Number of reflections | 79142 | |
<I/σ(I)> | 9.6 | 3.36 |
Completeness [%] | 99.7 | 99.7 |
Redundancy | 4.3 | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | 18% PEG 10k, 0.1M Tris, 0.2M CaCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |