2GRX
Crystal structure of TonB in complex with FhuA, E. coli outer membrane receptor for ferrichrome
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-06-29 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.932 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 234.320, 91.840, 138.510 |
| Unit cell angles | 90.00, 118.86, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.300 |
| R-factor | 0.299 |
| Rwork | 0.284 |
| R-free | 0.32900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fcp |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.676 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.510 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.084 | 0.566 |
| Number of reflections | 38697 | |
| <I/σ(I)> | 7.7 | 2.2 |
| Completeness [%] | 99.1 | 97.3 |
| Redundancy | 4.9 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | 8% polyethylene glycol 4000, 50 mM MES (pH 6.0), 75 mM NaCl, 5% glycerol, 0.7% C8E4, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
