2GQQ

Crystal Structure of E. coli Leucine-responsive regulatory protein (Lrp)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2001-06-01
Detector	MARRESEARCH
Wavelength(s)	1.08
Spacegroup name	C 2 2 21
Unit cell lengths	100.100, 237.000, 75.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 3.200
R-factor	0.322
Rwork	0.267
R-free	0.32200
Structure solution method	MIR
RMSD bond length	0.006
RMSD bond angle	1.100
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	3.270
High resolution limit [Å]	3.200	3.200
Number of reflections	15185
Completeness [%]	99.9	99.9
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	290	0.05M MES pH 5.6, 0.01M magnesium chloride, 0.4M potassium chloride, 9% PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 290K
1	VAPOR DIFFUSION, HANGING DROP	5.6	290	0.05M MES pH 5.6, 0.01M magnesium chloride, 0.4M potassium chloride, 9% PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 290K