2GMH
Structure of Porcine Electron Transfer Flavoprotein-Ubiquinone Oxidoreductase in Complexed with Ubiquinone
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-11-21 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0, 1.7389, 1.7426, 1.6000 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 154.322, 154.322, 128.536 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.670 - 2.500 |
| R-factor | 0.221 |
| Rwork | 0.221 |
| R-free | 0.25400 |
| Structure solution method | MAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 3.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.670 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 53508 | |
| <I/σ(I)> | 35.3 | 7.6 |
| Completeness [%] | 98.7 | 97.4 |
| Redundancy | 11.9 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | PEGMME 2000, Sodium Chloride, Ethylene glycol, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
