2GL8
Human Retinoic acid receptor RXR-gamma ligand-binding domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-03-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.070, 161.316, 68.682 |
| Unit cell angles | 90.00, 109.50, 90.00 |
Refinement procedure
| Resolution | 80.580 - 2.400 |
| R-factor | 0.24528 |
| Rwork | 0.243 |
| R-free | 0.29574 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rdt |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.475 |
| Data reduction software | CrystalClear ((MSC/RIGAKU)) |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.580 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 37858 | |
| Completeness [%] | 99.1 | 93.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 300 | peg, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
