2GJ9
Structure of the MnmE G-domain in complex with GDP*AlF4-, Mg2+ and Rb+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Detector technology | CCD |
| Collection date | 2005-04-18 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.815 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.240, 70.100, 90.210 |
| Unit cell angles | 90.00, 95.76, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.252 |
| Rwork | 0.250 |
| R-free | 0.29900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.860 |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.100 | |
| High resolution limit [Å] | 2.000 | 6.000 | 2.000 |
| Rmerge | 0.069 | 0.034 | 0.228 |
| Number of reflections | 48094 | 2687 | 11255 |
| <I/σ(I)> | 8.61 | 18.3 | 3.4 |
| Completeness [%] | 96.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 16-19% PEG 3350, 100 mM MES pH 6.0, 200 mM MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
