2GJ7

Crystal Structure of a gE-gI/Fc complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-1
Synchrotron site	SSRL
Beamline	BL9-1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2002-05-16
Detector	MAR scanner 345 mm plate
Wavelength(s)	0.9537
Spacegroup name	P 43 21 2
Unit cell lengths	174.726, 174.726, 315.997
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.000 - 5.000
Structure solution method	MR, MIRAS
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.000	5.270
High resolution limit [Å]	5.000	5.000
Rmerge	0.106	0.708
Number of reflections	21931
<I/σ(I)>	21.3	4.2
Completeness [%]	99.7	100
Redundancy	11.2	10.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	0.9-1.1 M sodium malonate, 0.1 M Hepes or 0.1 M MES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	0.9-1.1 M sodium malonate, 0.1 M Hepes or 0.1 M MES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	0.9-1.1 M sodium malonate, 0.1 M Hepes or 0.1 M MES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	0.9-1.1 M sodium malonate, 0.1 M Hepes or 0.1 M MES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	0.9-1.1 M sodium malonate, 0.1 M Hepes or 0.1 M MES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	0.9-1.1 M sodium malonate, 0.1 M Hepes or 0.1 M MES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K