2GJ4
Structure of rabbit muscle glycogen phosphorylase in complex with ligand
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX9.6 |
Synchrotron site | SRS |
Beamline | PX9.6 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-10-24 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.87 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 128.128, 128.128, 116.346 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.800 - 1.600 |
R-factor | 0.1889 |
Rwork | 0.187 |
R-free | 0.22616 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | In house model |
RMSD bond length | 0.009 |
RMSD bond angle | 1.184 |
Data reduction software | MOSFLM |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.800 | 1.690 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.059 | 0.466 |
Number of reflections | 94754 | |
<I/σ(I)> | 12 | 1.5 |
Completeness [%] | 74.5 | 74.5 |
Redundancy | 3.3 | 1.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.7 | 289 | 1.1mM IMP, 1.1mM Spermine, 0mM BES, 2.9mM DTT, 0.1mM EDTA, pH 6.7, VAPOR DIFFUSION, SITTING DROP, temperature 289K |