2GIC
Crystal Structure of a vesicular stomatitis virus nucleocapsid-RNA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-10-27 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.99997 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 166.160, 236.316, 75.651 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.860 - 2.920 |
| R-factor | 0.258 |
| Rwork | 0.255 |
| R-free | 0.30600 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.153 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.860 | 3.020 |
| High resolution limit [Å] | 2.920 | 2.920 |
| Number of reflections | 59391 | |
| Completeness [%] | 97.0 | 91.86 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 7 % PEG 3350, 250 mM sodium chloride, 100mM sodium acetate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 3350 | ||
| 2 | 1 | 1 | sodium chloride | ||
| 3 | 1 | 1 | sodium acetate | ||
| 4 | 1 | 1 | H2O | ||
| 5 | 1 | 2 | PEG 3350 | ||
| 6 | 1 | 2 | sodium chloride | ||
| 7 | 1 | 2 | sodium acetate | ||
| 8 | 1 | 2 | H2O |
