2GGM
Human centrin 2 xeroderma pigmentosum group C protein complex
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12B |
| Synchrotron site | NSLS |
| Beamline | X12B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-26 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.8856 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 59.150, 59.000, 104.240 |
| Unit cell angles | 90.00, 94.30, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.350 |
| R-factor | 0.19439 |
| Rwork | 0.192 |
| R-free | 0.24535 |
| Structure solution method | MAD |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.139 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.390 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.082 | 0.560 |
| Number of reflections | 15095 | |
| <I/σ(I)> | 18 | 1.74 |
| Completeness [%] | 99.3 | 96.3 |
| Redundancy | 6.6 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 2.5-3.2 M (NH4)2SO4, 0.1-0.4 M Na2HPO4, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
