2GFE
Crystal structure of the GluR2 A476E S673D Ligand Binding Core Mutant at 1.54 Angstroms Resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-10-13 |
Detector | MARRESEARCH |
Wavelength(s) | 1.00000 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 114.318, 163.301, 47.557 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.420 - 1.540 |
R-factor | 0.2183 |
Rwork | 0.212 |
R-free | 0.22740 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 1ftj |
RMSD bond length | 0.004 |
RMSD bond angle | 1.154 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.600 |
High resolution limit [Å] | 1.540 | 1.540 |
Rmerge | 0.049 | 0.354 |
Number of reflections | 132397 | |
<I/σ(I)> | 15 | 2.8 |
Completeness [%] | 98.2 | 97.2 |
Redundancy | 3.6 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 277 | 1 to 1 mixture of: Reservoir: 14% PEG 8K; 0.1M Na Acetate; 0.1 M Zn Acetate. Protein: 10 mg/ml 0.01 M Na HEPES pH 7.0; 0.02 M NaCl; 0.01 M Na Glutamate; 0.001 M EDTA, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |