2GDF
Crystal structure of Dioclea violacea seed lectin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM16 |
| Synchrotron site | ESRF |
| Beamline | BM16 |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2004-09-20 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97944 |
| Spacegroup name | P 32 |
| Unit cell lengths | 72.938, 72.938, 161.362 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.000 - 2.400 |
| R-factor | 0.22823 |
| Rwork | 0.227 |
| R-free | 0.25785 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.651 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.000 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 37630 | |
| Completeness [%] | 100.0 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 10% PEG 8000, 10% PEG 1000, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
