2GDC
Structure of Vinculin VD1 / IpaA560-633 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-10-22 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.8423 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 52.684, 68.326, 96.521 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.180 - 2.740 |
| R-factor | 0.24966 |
| Rwork | 0.247 |
| R-free | 0.29400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1syq |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.578 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (refmac_5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 15.190 | 41.740 | 2.890 |
| High resolution limit [Å] | 2.740 | 8.660 | 2.740 |
| Rmerge | 0.085 | 0.030 | 0.350 |
| Number of reflections | 9559 | ||
| <I/σ(I)> | 7.1 | 7 | 2.1 |
| Completeness [%] | 99.0 | ||
| Redundancy | 4.7 | 4.2 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 100 mM HEPES, 10% 2-propanol, 20% PEG 4000, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
