2GCO
Crystal structure of the human RhoC-GppNHp complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-04 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 64.092, 75.645, 82.903 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.400 |
| R-factor | 0.19804 |
| Rwork | 0.197 |
| R-free | 0.21900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1z2c |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.360 |
| Data reduction software | CrystalClear ((MSC/RIGAKU)) |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 82.903 | 1.480 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.085 | 0.508 |
| Total number of observations | 52822 | |
| Number of reflections | 75377 | 11027 |
| <I/σ(I)> | 4.3 | 1.2 |
| Completeness [%] | 95.0 | 95 |
| Redundancy | 5 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 291.15 | 30% PEG 8000, 0.2M Sodium Acetate,1.2% Inositol, 0.1M Sodium Cacodylate, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 291.15K |
