2GC7
Substrate reduced, copper free complex of methylamine dehydrogenase, amicyanin and cytochrome c551i from Paracoccus denitrificans.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 298 |
| Detector technology | DIFFRACTOMETER |
| Collection date | 1996-05-25 |
| Detector | WEISSENBERG |
| Wavelength(s) | 0.95 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 81.323, 189.127, 128.906 |
| Unit cell angles | 90.00, 99.75, 90.00 |
Refinement procedure
| Resolution | 29.940 - 1.900 |
| Rwork | 0.171 |
| R-free | 0.19800 |
| Structure solution method | Refined directly |
| Starting model (for MR) | 2gc4 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.055 | 0.276 |
| Number of reflections | 254385 | |
| <I/σ(I)> | 12.4 | 2.2 |
| Completeness [%] | 83.2 | 44.8 |
| Redundancy | 1.8 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 295 | 2.3-2.6M sodium/potassium phosphate, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
