2GC6
S73A mutant of L. casei FPGS
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 110 |
Detector technology | IMAGE PLATE |
Detector | MARRESEARCH |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 53.120, 45.860, 84.270 |
Unit cell angles | 90.00, 106.97, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.900 |
R-factor | 0.193 |
Rwork | 0.191 |
R-free | 0.23500 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.370 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.060 | 0.292 |
Number of reflections | 29157 | |
<I/σ(I)> | 21 | 3.8 |
Completeness [%] | 96.1 | 93.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.3 | 277 | 50 mM acetate and 16-24% PEG4000, pH 5.3, VAPOR DIFFUSION, HANGING DROP, temperature 277K |