2GBW
Crystal Structure of Biphenyl 2,3-Dioxygenase from Sphingomonas yanoikuyae B1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-07-24 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 134.958, 134.958, 219.887 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.800 - 1.700 |
| R-factor | 0.19012 |
| Rwork | 0.188 |
| R-free | 0.22830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ndo |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.629 |
| Data scaling software | d*TREK (9.1L) |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.800 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.083 | 0.394 |
| Number of reflections | 241600 | |
| <I/σ(I)> | 8.2 | 2.9 |
| Completeness [%] | 95.3 | 98.1 |
| Redundancy | 4.48 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 277.15 | 20% PEG3350, 1.0 M Sodium Chloride, 0.037M Zinc Chloride, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 277.15K |
