2GAZ
Mycobacterial lipoglycan presentation by CD1d
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-07-06 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 41.835, 110.757, 107.396 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.340 - 2.610 |
R-factor | 0.21566 |
Rwork | 0.212 |
R-free | 0.28032 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2akr |
RMSD bond length | 0.015 |
RMSD bond angle | 1.745 |
Data reduction software | HKL-2000 |
Data scaling software | CCP4 ((TRUNCATE)) |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.690 |
High resolution limit [Å] | 2.600 | 2.600 |
Number of reflections | 15322 | |
<I/σ(I)> | 7.9 | 1.8 |
Completeness [%] | 97.0 | 98.5 |
Redundancy | 3 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 277 | 20% Peg 4000, 0.1M sodium citrate, 10% n-propanol, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 277.0K |