2GAM
X-ray crystal structure of murine leukocyte-type Core 2 b1,6-N-acetylglucosaminyltransferase (C2GnT-L) in complex with Galb1,3GalNAc
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2003-07-12 |
| Detector | SBC-3 |
| Wavelength(s) | 1.0729 |
| Spacegroup name | P 1 |
| Unit cell lengths | 73.498, 80.585, 102.325 |
| Unit cell angles | 73.61, 76.26, 64.56 |
Refinement procedure
| Resolution | 20.000 - 2.700 |
| R-factor | 0.217 |
| Rwork | 0.217 |
| R-free | 0.27400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gak |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.130 | 0.660 |
| Number of reflections | 69466 | |
| <I/σ(I)> | 12.1 | 1.5 |
| Completeness [%] | 96.2 | 78.3 |
| Redundancy | 4.3 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 298 | 20% PEG 4000, 0.1M glycine pH=9.0, 0.6M lithium chloride, 3.3% 1,2,3 heptanetriol, 20mM Galb1,3GalNAc, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
