2GAI
Structure of Full Length Topoisomerase I from Thermotoga maritima in triclinic crystal form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-26 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9756 |
| Spacegroup name | P 1 |
| Unit cell lengths | 45.118, 95.421, 96.508 |
| Unit cell angles | 83.40, 86.15, 84.87 |
Refinement procedure
| Resolution | 41.560 - 1.700 |
| R-factor | 0.199 |
| Rwork | 0.197 |
| R-free | 0.23200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ecl |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.758 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.560 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.070 | 0.440 |
| Number of reflections | 167812 | |
| <I/σ(I)> | 10.5 | 3 |
| Completeness [%] | 95.6 | 95.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 291 | 33% Jeffamine M-600, 100 mM sodium citrate/HCl, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
