2G9G
Crystal structure of His-tagged mouse PNGase C-terminal domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X26C |
Synchrotron site | NSLS |
Beamline | X26C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-06-29 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9996 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 71.732, 40.747, 66.506 |
Unit cell angles | 90.00, 95.34, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.000 |
R-factor | 0.15304 |
Rwork | 0.150 |
R-free | 0.20711 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2g2f |
RMSD bond length | 0.014 |
RMSD bond angle | 1.495 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.108 | 0.485 |
Number of reflections | 13124 | |
<I/σ(I)> | 17.8 | 3.9 |
Completeness [%] | 99.9 | 99.4 |
Redundancy | 4.6 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 1.6 M Li2SO4 and 100 mM Hepes, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |