2G95

Crystal Structure of Visfatin/Pre-B Cell Colony Enhancing Factor 1/Nicotinamide Phosphoribosyltransferase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 4A
Synchrotron site	PAL/PLS
Beamline	4A
Temperature [K]	100
Detector technology	CCD
Collection date	2005-06-26
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9795
Spacegroup name	P 1 21 1
Unit cell lengths	66.300, 94.917, 84.866
Unit cell angles	90.00, 104.91, 90.00

Refinement procedure

Resolution	20.000 - 1.900
R-factor	0.238
Rwork	0.235
R-free	0.26700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	the Se-Met Phasing using other crystal
RMSD bond length	0.006
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.930
High resolution limit [Å]	1.900	1.900
Number of reflections	80015
Completeness [%]	99.9	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	298	0.1M HEPES-NaOH at pH 7.2, 13-16% PEG3350, and 4-7% t-butanol, VAPOR DIFFUSION, HANGING DROP, temperature 298K