2G8S
Crystal structure of the soluble Aldose sugar dehydrogenase (Asd) from Escherichia coli in the apo-form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-12-11 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97935 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.829, 113.475, 75.372 |
| Unit cell angles | 90.00, 90.03, 90.00 |
Refinement procedure
| Resolution | 7.960 - 1.500 |
| R-factor | 0.148 |
| Rwork | 0.149 |
| R-free | 0.18300 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.215 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MLPHARE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.378 | 1.380 |
| High resolution limit [Å] | 1.315 | 1.310 |
| Rmerge | 0.057 | 0.386 |
| Total number of observations | 4343 | |
| Number of reflections | 162184 | 2572 |
| <I/σ(I)> | 6.9 | 1.9 |
| Completeness [%] | 72.2 | 10.9 |
| Redundancy | 3.3 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.2 | 289 | 18 % PEG 6000, 100 mM CHES, 100 mM sodium chloride, 1 mM calcium chloride, pH 9.2, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
