2G8H

B. halodurans RNase H catalytic domain D192N mutant in complex with Mg2+ and RNA/DNA hybrid (non-P nick at the active site)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	95
Detector technology	IMAGE PLATE
Collection date	2005-01-01
Detector	RIGAKU RAXIS IV
Spacegroup name	C 1 2 1
Unit cell lengths	82.441, 36.964, 61.810
Unit cell angles	90.00, 96.38, 90.00

Refinement procedure

Resolution	30.000 - 1.850
Rwork	0.206
R-free	0.25400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1zbi
RMSD bond length	0.005
RMSD bond angle	1.120
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	EPMR
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.920
High resolution limit [Å]	1.850	1.850
Number of reflections	14882
Completeness [%]	92.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	278	32% MPD, 0.1M Tris pH 7.0, 0.2 M NaCl, VAPOR DIFFUSION, SITTING DROP, temperature 278K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	MPD
2	1	1	Tris
3	1	1	NaCl
4	1	1	H2O
5	1	2	MPD
6	1	2	Tris
7	1	2	NaCl