2G8H
B. halodurans RNase H catalytic domain D192N mutant in complex with Mg2+ and RNA/DNA hybrid (non-P nick at the active site)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 95 |
Detector technology | IMAGE PLATE |
Collection date | 2005-01-01 |
Detector | RIGAKU RAXIS IV |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 82.441, 36.964, 61.810 |
Unit cell angles | 90.00, 96.38, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.850 |
Rwork | 0.206 |
R-free | 0.25400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1zbi |
RMSD bond length | 0.005 |
RMSD bond angle | 1.120 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | EPMR |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.920 |
High resolution limit [Å] | 1.850 | 1.850 |
Number of reflections | 14882 | |
Completeness [%] | 92.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 278 | 32% MPD, 0.1M Tris pH 7.0, 0.2 M NaCl, VAPOR DIFFUSION, SITTING DROP, temperature 278K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | MPD | ||
2 | 1 | 1 | Tris | ||
3 | 1 | 1 | NaCl | ||
4 | 1 | 1 | H2O | ||
5 | 1 | 2 | MPD | ||
6 | 1 | 2 | Tris | ||
7 | 1 | 2 | NaCl |