2G78
Crystal Structure of the R132K:Y134F Mutant of Cellular Retinoic Acid Binding Protein Type II in Complex with All-Trans-Retinoic Acid at 1.70 Angstroms Resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 32-ID |
Synchrotron site | APS |
Beamline | 32-ID |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2003-01-12 |
Detector | MARRESEARCH |
Wavelength(s) | 1.000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 44.702, 46.251, 77.553 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.720 - 1.700 |
R-factor | 0.151 |
Rwork | 0.145 |
R-free | 0.20300 |
Structure solution method | Rigid Body Refinement |
RMSD bond length | 0.017 |
RMSD bond angle | 1.675 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.046 | 0.361 |
Number of reflections | 17545 | 1085 |
<I/σ(I)> | 17.66 | 1.91 |
Completeness [%] | 95.6 | 60.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 298 | 0.1 M sodium citrate pH 4.8, 20% PEG 5000 and 10% DMSO, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 298 | 0.1 M sodium citrate pH 4.8, 20% PEG 5000 and 10% DMSO, VAPOR DIFFUSION, HANGING DROP, temperature 298K |