2G64
Structure of Caenorhabditis elegans 6-pyruvoyl tetrahydropterin synthase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2006-01-31 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | H 3 2 |
Unit cell lengths | 69.792, 69.792, 160.188 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 1.800 |
R-factor | 0.16238 |
Rwork | 0.161 |
R-free | 0.21902 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1b66 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.251 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.031 | 0.094 |
Number of reflections | 14091 | |
<I/σ(I)> | 38.9 | 14.6 |
Completeness [%] | 98.7 | 97.9 |
Redundancy | 10.4 | 5.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 290 | 100 MM HEPES, 1 M SODIUM SUCCINATE, PH 7.00, 1% PEG 2000ME, VAPOR DIFFUSION, SITTING DROP, temperature 290K |