2G4G
Crystal structure of human transthyretin at pH 4.6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-12-04 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.2 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 42.597, 85.953, 63.688 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.600 - 1.850 |
| R-factor | 0.212 |
| Rwork | 0.207 |
| R-free | 0.23300 |
| Structure solution method | MIR |
| Starting model (for MR) | 1f41 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.049 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.600 | 1.950 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Number of reflections | 17809 | |
| <I/σ(I)> | 12.4 | 2 |
| Completeness [%] | 87.9 | 87.7 |
| Redundancy | 4.9 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | 15% PEG monomethyl ether, 0.1M ammonium sulphate, 50mM sodium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
