2G3W
The Crystal Structure of YaeQ Protein from Xanthomonas axonopodis pv. citri
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-07-29 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97952, 0.97962, 1.00000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 39.766, 91.969, 48.031 |
Unit cell angles | 90.00, 108.35, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.900 |
R-factor | 0.194 |
Rwork | 0.192 |
R-free | 0.24700 |
Structure solution method | MAD |
RMSD bond length | 0.015 |
RMSD bond angle | 1.481 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 24538 | |
<I/σ(I)> | 33.7 | 3 |
Completeness [%] | 94.8 | 86.1 |
Redundancy | 4 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 291 | 0.1M Tris pH 9, 0.2M ammonium acetate, 32% PEG 4000, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |