2G1H

Structure of E.coli FabD complexed with glycerol

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ENRAF-NONIUS FR591
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2001-07-13
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	P 41 21 2
Unit cell lengths	83.147, 83.147, 164.511
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.860
R-factor	0.21111
Rwork	0.211
R-free	0.22213
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1mla
RMSD bond length	0.005
RMSD bond angle	0.754
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.980
High resolution limit [Å]	1.860	1.860
Number of reflections	48061
<I/σ(I)>	44.7
Completeness [%]	97.5	99.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1