2G0B
The structure of FeeM, an N-acyl amino acid synthase from uncultured soil microbes
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 8-BM |
Synchrotron site | APS |
Beamline | 8-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-07-16 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9795, 0.9422, 0.9793 |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 182.832, 182.832, 287.429 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.750 - 3.000 |
Rwork | 0.255 |
R-free | 0.29200 |
Structure solution method | MAD |
RMSD bond length | 0.011 |
RMSD bond angle | 0.917 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (CCP4_3.1.20) |
Phasing software | SHARP |
Refinement software | REFMAC (refmac_5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 129.100 | 3.150 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.081 | 0.160 |
Total number of observations | 110846 | |
Number of reflections | 48895 | |
<I/σ(I)> | 7.8737 | 1.31 |
Completeness [%] | 100.0 | 100 |
Redundancy | 16.4 | 16.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 10 | 289 | 4.06 M NaCl, 50 mM glycine, pH 10.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K |