2FZE
Crystal structure of the binary complex of human glutathione-dependent formaldehyde dehydrogenase with ADP-ribose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 113 |
Wavelength(s) | 1.2820 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 79.234, 79.234, 310.964 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.600 - 1.900 |
Rwork | 0.183 |
R-free | 0.20900 |
Structure solution method | SAD |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE (2.06) |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.098 | 0.434 |
Number of reflections | 79380 | 7762 |
<I/σ(I)> | 14.8 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 27.2 | 23 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.1 | 277 | 15% PEG 8000, 100 mM potassium phosphate, 100 uM zinc sulphate, pH 7.1, VAPOR DIFFUSION, SITTING DROP, temperature 277K |