2FYF

Structure of a putative phosphoserine aminotransferase from Mycobacterium Tuberculosis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	CCD
Collection date	2005-09-25
Detector	ADSC QUANTUM 210
Wavelength(s)	1.07198
Spacegroup name	P 21 21 21
Unit cell lengths	77.478, 94.056, 101.063
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.932 - 1.500
R-factor	0.145
Rwork	0.144
R-free	0.16640
Structure solution method	MIR
RMSD bond length	0.013
RMSD bond angle	1.487
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	REFMAC (refmac_5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	1.550
High resolution limit [Å]	1.500	1.500
Rmerge	0.086	0.559
Number of reflections	118835	11589
<I/σ(I)>	17.38	2.6
Completeness [%]	99.7	98.1
Redundancy	5	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	291	Tris-HCl 100mM, NH4SO4 2.2M, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K
1	VAPOR DIFFUSION, SITTING DROP	8.5	291	Tris-HCl 100mM, NH4SO4 2.2M, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K
1	VAPOR DIFFUSION, SITTING DROP	8.5	291	Tris-HCl 100mM, NH4SO4 2.2M, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K