2FXO
Structure of the human beta-myosin S2 fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-02-16 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 |
| Unit cell lengths | 40.157, 41.867, 97.795 |
| Unit cell angles | 91.11, 92.73, 107.18 |
Refinement procedure
| Resolution | 20.000 - 2.500 |
| R-factor | 0.277 |
| Rwork | 0.273 |
| R-free | 0.34900 |
| Structure solution method | SAD WITH SEMI-BRUTE- FORCE MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1XNM |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.526 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHELXD |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 20190 | |
| <I/σ(I)> | 9 | 3.2 |
| Completeness [%] | 96.0 | 96.6 |
| Redundancy | 3.2 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 296 | PEG 3350, SODIUM ACETATE, TRIS-HCL, pH 7.50, VAPOR DIFFUSION, SITTING DROP, temperature 296K |
