2FX3
Crystal Structure Determination of E. coli Elongation Factor, Tu using a Twinned Data Set
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2004-08-24 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.033 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 69.550, 69.550, 169.440 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 60.232 - 3.400 |
Rwork | 0.197 |
R-free | 0.26190 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1d8t chain A |
RMSD bond length | 0.011 |
RMSD bond angle | 1.730 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 60.232 | 3.580 |
High resolution limit [Å] | 3.400 | 3.400 |
Rmerge | 0.174 | 0.480 |
Total number of observations | 5800 | |
Number of reflections | 6312 | 844 |
<I/σ(I)> | 3.2 | 1.5 |
Completeness [%] | 90.2 | 84.9 |
Redundancy | 6.6 | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.8 | 293 | 0.45 mM EF-Tu in 50 mM Tris, pH 7.8, 10 mM GDP, 10 mM magnesium chloride, 0.5% PEG 3350, 5.5 mM ammonium acetate, 2.7 mM ammonium citrate, 1.34 mM ChemDiv compound 1013-0135, VAPOR DIFFUSION, SITTING DROP, temperature 293K |