2FX3

Crystal Structure Determination of E. coli Elongation Factor, Tu using a Twinned Data Set

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	95
Detector technology	CCD
Collection date	2004-08-24
Detector	ADSC QUANTUM 4
Wavelength(s)	1.033
Spacegroup name	P 31 2 1
Unit cell lengths	69.550, 69.550, 169.440
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	60.232 - 3.400
Rwork	0.197
R-free	0.26190
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1d8t chain A
RMSD bond length	0.011
RMSD bond angle	1.730
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.232	3.580
High resolution limit [Å]	3.400	3.400
Rmerge	0.174	0.480
Total number of observations		5800
Number of reflections	6312	844
<I/σ(I)>	3.2	1.5
Completeness [%]	90.2	84.9
Redundancy	6.6	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.8	293	0.45 mM EF-Tu in 50 mM Tris, pH 7.8, 10 mM GDP, 10 mM magnesium chloride, 0.5% PEG 3350, 5.5 mM ammonium acetate, 2.7 mM ammonium citrate, 1.34 mM ChemDiv compound 1013-0135, VAPOR DIFFUSION, SITTING DROP, temperature 293K